Table 1.
Top ten biological processes identified from 507 gene ontology terms summarized by REVIGO in A–F1 versus N–F1
| GO ID | Description | Log10 P value | Genes |
|---|---|---|---|
| 0022412 | Cellular process involved in reproduction in multicellular organism | −31.29 | ↓DDX20, ↓CEP57, ↓PCSK4, ↓KDM3A, ↓CFTR, ↓MTOR, ↓PRKDC, ↓STK11, ↓ZPBP2, ↓PDILT,
↓PDE3A, ↓SRPK1, ↓SPINK2, ↓SPAM1, ↓TTLL5, ↓HORMAD1, ↓NEURL1, ↓KLHL10, ↓OCA2, ↓CATSPERD, ↓TGFBR1, ↓TSSK2, ↓PIWIL1, ↓DZIP1, ↓NUP210L, ↓NPHP1, ↓AGFG1, ↓AFF4, ↓TMF1, ↓SERPINA5, ↓GALNTL5, ↓TRIP13, ↓MOV10L1, ↓PSME4, ↓SYCP1, ↓RNF17, ↓IFT88, ↓DIAPH 2, ↓STRBP, ↓ADAM2, ↓CASC5, ↓CRISP1, ↓SMAD5, ↓CABYR |
| 0048515 | Spermatid differentiation | −27.95 | ↓CEP57, ↓PCSK4, ↓KDM3A, ↓CFTR, ↓STK11, ↓ZPBP2, ↓PDILT, ↓SRPK1, ↓SPINK2, ↓TTLL5,
↓NEURL1, ↓KLHL10, ↓OCA2, ↓CATSPERD, ↓TSSK2, ↓PIWIL1, ↓NUP210L, ↓NPHP1, ↓AGFG1, ↓AFF4, ↓TMF1, ↓GALNTL5, ↓TRIP13, ↓PSME4, ↓SYCP1, ↓RNF17, ↓IFT88, ↓STRBP, ↓CASC5, ↓CABYR |
| 0007286 | Spermatid development | −27.37 | ↓CEP57, ↓PCSK4, ↓KDM3A, ↓CFTR, ↓STK11, ↓ZPBP2, ↓PDILT, ↓SRPK1, ↓SPINK2, ↓TTLL5,
↓NEURL1, ↓KLHL10, ↓OCA2, ↓CATSPERD, ↓TSSK2, ↓PIWIL1, ↓NUP210L, ↓AGFG1, ↓AFF4, ↓TMF1, ↓GALNTL5, ↓TRIP13, ↓PSME4, ↓SYCP1, ↓RNF17, ↓IFT88, ↓STRBP, ↓CASC5, ↓CABYR |
| 0060271 | Cilium assembly | −19.88 | ↓KIAA0586, ↓C2CD3, ↓ZMYND10, ↓RFX3, ↓NEK1, ↓FBF1, ↓ARL13B, ↓TTLL5, ↓LRRC6,
c ↓NEURL1, ↓CELSR2, ↓ATP6V0D1, ↓DNAAF1, ↓DZIP1, ↓TMEM67, ↓TTBK2, ↓AHI1, ↓PCM1, ↓ABLIM1, ↓IFT74, ↓IFT80, ↓IFT81, ↓IFT88, ↓TRAF3IP1, ↓TCTN2, ↓FOPNL, ↓CEP164, ↓ACTR2, ↓RPGRIP1L, ↓KIF3A, ↓KIF24, ↓KIF27 |
| 0007018 | Microtubule-based movement | −19.24 | ↓RHOT1, ↓KIF21B, ↓KIF16B, ↓KIFAP3, ↓NDE1, ↓RFX3, ↓NEFM, ↓ARMC4, ↓CDC42, ↓MAP1S,
↓TTLL6, ↓LRRC6, ↓CELSR2, ↓DNAAF1, ↓KTN1, ↓RAB1A, ↓DNAH9, ↓DNAH3, ↓DNAH1, ↓DNAH7, ↓DNAH8, ↓DNAH12, ↓DNAH14, ↓PCM1, ↓IFT74, ↓IFT81, ↓TRAF3IP1, ↓AP3D1, ↓KIFC3, ↓ACTR3, ↓ACTR2, ↓KIF3A, ↓KIF24, ↓KIF27, ↓KIF2C, ↓KIF2A, ↓CABYR |
| 0009566 | Fertilization | −18.44 | ↓PCSK4, ↓TUBGCP3, ↓CLGN, ↓ZPBP2, ↓CCT3, ↓CCT4, ↓SPINK2, ↓SPAM1, ↓TTLL5, ↓KLHL10,
↓CATSPERD, ↓CATSPERG, ↓ATP8B3, ↓TDRD9, ↓ARSA, ↓PLCZ1, ↓WDR48, ↓PLCD4, ↓ADAM21, ↓SPACA3, ↓SERPINA5, ↓TRIM36, ↓MAEL, ↓SYCP2, ↓TEX15, ↓ADAM2, ↓UBE3A, ↓CRISP1, ↓CACNA1H |
| 0000209 | Protein polyubiquitination | −12.58 | ↓WWP2, ↓HACE1, ↓ITCH, ↓BTRC, ↓CDC27, ↓UBE2G1, ↓UBE2R2, ↓SMURF1, ↓DTL, ↓HUWE1,
↓DZIP3, ↓TPP2, ↓AMFR, ↓TRIP12, ↓RNF216, ↓PSMB1, ↓PSMD3, ↓PSMD6, ↓PSMD8, ↓PSME4, ↓PSMF1, ↓RBCK1, ↓RNF167, ↓RNF111, ↓UBE2K, ↓UBE3A, ↓UBE3C, ↓SHARPIN, ↓RC3H2 |
| 0001701 | In utero embryonic development | −12.51 | ↓MED1, ↓C2CD3, ↓MKL2, ↓EPB41L5, ↓FLVCR1, ↓TAPT1, ↓HORMAD1, ↓SOX6, ↓EPN1, ↓STIL,
↓PRKCSH, ↓BRK1, ↓NCOA1, ↓NCOA6, ↓BPTF, ↓TGFBR1, ↓WDTC1, ↓COL4A3BP, ↓FUT8, ↓SP2, ↓EPAS1, ↓BIRC6, ↓UBR3, ↓SEC24D, ↓DUSP3, ↓RPGRIP1L, ↓SMAD4, ↓SMAD2, ↓KIF3A, ↓INTS1 |
| 0035036 | Sperm–egg recognition | −11.52 | ↓PCSK4, ↓CLGN, ↓ZPBP2, ↓CCT3, ↓CCT4, ↓SPAM1, ↓CATSPERD, ↓CATSPERG, ↓ATP8B3, ↓ARSA,
↓ADAM21, ↓SPACA3, ↓ADAM2, ↓CRISP1 |
| 0007030 | Golgi organization | −9.90 | ↓NPLOC4, ↓HACE1, ↓BLZF1, ↓BCAS3, ↓TMED5, ↓GOLGA5, ↓ARFGEF1, ↓PLEKHM2, ↓CDC42,
↓USP6NL, ↓ATL3, ↓DYM, ↓GCC2, ↓RAB1A, ↓KIFC3 |
Arrows (↓) show gene expression directionality. The biological processes summarized by REVIGO (http://revigo.irb.hr) were generated with FatiGO (http://babelomics.bioinfo.cipf.es) using the list of 1863 DEGs. GO ID: gene ontology identification code. A-F1: asthenozoospermic sperm subpopulation with high motility; N-F1: normozoospermic sperm subpopulation with high motility