Figure 1.

Schematic diagram of the one-step method used for isolation and culture of ADSCs and DAs from adipose tissues. Following collagenase digestion and centrifugation the fat cell layer was transferred a TC-treated dish and a TC untreated dish cover was placed in it, so that the floating fat cells could attach. After 10–14 days, spindle cells grew on the bottom dish treated with TC, which were then passaged regularly to acquire the DAs, and ADSCs were obtained via the conventional culture of SVF. TC, tissue culture; ADSCs, adipose-derived stem cells; DAs, dedifferentiated adipose cells; SVF, stromal vascular fraction.