Figure 2.

Basic characteristics of ADSCs and DAs. (A) Newly inoculated adipocytes, primary DAs, and primary ADSCs are shown successively from left to right. Scale bar, 200 µm. (B) ADSCs and DAs of 6–7 passages had similar spindle shapes without lipid droplets. Scale bar, 100 µm. (C) Cell proliferation curves showed notably individual differences in growth speed, while no definite patterns of growth speed were observed between the two types of cells. *P<0.05. (D) FACS results of three pairs of cells. The red histogram represents isotype control, and the blue histograms represents the detected markers. (E) Summary of the immunophenotype of six cell types demonstrated no significant differences in the expression of the six markers between the two groups. MA, mature adipocytes; ADSCs, adipose-derived stem cells; DAs, dedifferentiated adipose cells; FACS, fluorescent activated cell sorting.