Table 2.
Primer sequences, fragment size, and PCR conditions for detection of virulence genes in Pseudomonas aeruginosa
| Gene | Primer sequence (5′➔3′) | Fragment size (bp) | Annealing T (°C) |
|---|---|---|---|
| algD | F: AAGGCGGAAATGCCATCTCC | 290 | 60 |
| R: AGGGAAGTTCCGGGCGTTTG | |||
| exoY | F: TATCGACGGTCATCGTCAGGT | 1035 | |
| R: TTGATGCACTCGACCAGCAAG | |||
| plcH | F: GCACGTGGTCATCCTGATGC | 608 | |
| R: TCCGTAGGCGTCGACGTAC | |||
| rhIR | F: TGCATTTTATCGATCAGGGC | 133 | 57 |
| R: CACTTCCTTTTCCAGGACG | |||
| lasI | F: CGTGCTCAAGTGTTCAAGG | 295 | |
| R: TACAGTCGGAAAAGCCCAG | |||
| apr | F: TGTCCAGCAATTCTCTTGC | 1017 | |
| R: CGTTTTCCACGGTGACC | |||
| exoT | F: CAATCATCTCAGCAGAACCC | 1159 | |
| R: TGTCGTAGAGGATCTCCTG | |||
| rhII | F: TTCATCCTCCTTTAGTCTTCCC | 155 | |
| R: TTCCAGCGATTCAGAGAGC | |||
| exoS | F: CGTCGTGTTCAAGCAGATGGTGCTG | 444 | 65 |
| R: CCGAACCGCTTCACCAGGC | |||
| plcN | F: TCCGTTATCGCAACCAGCCCTACG | 481 | |
| R: TCGCTGTCGAGCAGGTCGAAC | |||
| lasB | F: GGAATGAACGAAGCGTTCTCCGAC | 284 | |
| R: TGGCGTCGACGAACACCTCG | |||
| lasR | F: AAGTGGAAAATTGGAGTGGAG | 130 | |
| R: GTAGTTGCCGACGACGATGAAG | |||
| algU | F: CGCGAACCGCACCATCGCTC | 1500 | 66 |
| R: GCCGCACGTCACGAGC | |||
| exoU | F: TACCAGGTACGGCCATGTTC | 575 | 59 |
| R: ACGCTCTGAAGCCTGAAGAC |
Bp Base pairs; F Forward; R Reverse; T Temperature
PCR was performed following the manufacturer’s instructions