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. 2020 Nov 30;20:539. doi: 10.1186/s12870-020-02742-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Quantitative RT-PCR validation of seven differentially expressed R-gene transcripts from gerbera transcriptome analysis. These transcripts were differentially upregulated in powdery mildew resistant (R) line (UFGE 4033) versus powdery mildew-susceptible (S) line (06–245-03). Expression of transcripts were normalized to the endogenous control Gerbera hybrida actin gene and the relative expression was calculated using 2{−Ct (gene of interest)-Ct (actin)}. The blue line shows the Log2 fold change expression of the transcripts from RNA-seq data. Error bars represent standard errors