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. 2020 Dec 1;39:269. doi: 10.1186/s13046-020-01744-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — miR-144 can specifically bind to circ_001287. a, Binding sites between circ_001287 and miR-144 predicted through a bioinformatics website. b, Binding relationship of miR-144 to circ_001287 measured using dual-luciferase reporter gene assay. c, Binding of circ_001287 and Ago2 measured using RIP assay. d, Binding of miR-144 and Ago2 measured using RIP assay. e, Binding of circ_001287 and miR-144 analyzed using RNA pull-down assay. f, Co-location of circ_001287 and miR-144 in the cytoplasm tested using FISH (× 400). # p < 0.05 vs. the cells treated with mimic-NC; ^*p < 0.05 vs. IgG and NC-bio-probe. Statistical data were measurement data and described as mean ± standard deviation. Independent sample t-test was used for data comparison between the two groups. Data among groups were compared with one-way ANOVA together with Tukey’s post hoc test. The cell experiment was repeated 3 times. circRNA, circular RNA; miR-144, microRNA-144; Ago2, Argonaut-2; RIP, RNA immunoprecipitation; FISH, Fluorescence in situ hybridization