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. 2020 Dec 1;39:269. doi: 10.1186/s13046-020-01744-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — circ_001287 modulates miR-144-targeted CEP55 and thus hinders the progression of RCC. a, Binding sites between miR-144 and CEP55 predicted through a bioinformatics website. b, Targeting relationship between miR-144 and CEP55 tested by dual-luciferase reporter gene assay. c, Expression of CEP55 in RCC tissues and adjacent normal tissues measured using RT-qPCR (n = 77). d, Expression of CEP55 in RCC tissues and adjacent normal tissues measured using immunohistochemistry (× 200). e, Correlation analysis of circ_001287 and CEP55. f, Correlation analysis of miR-144 and CEP55. The 786-O cells were treated with oe-NC, oe-CEP55, or si-circ_001287 + oe-CEP55. g, mRNA expression of CEP55 in 786-O cells determined using RT-qPCR. h, Protein expression of CEP55 in 786-O cells tested using western blot analysis. i, Viability of 786-O cells measured using CCK-8 assay. j, Cell cycle distribution measured using flow cytometry. k, Image (× 200) of migration of 786-O cells measured using Transwell assay. l, Quantification analysis of migration ability of 786-O cells measured using Transwell assay. m, Image (× 200) of invasion of 786-O cells evaluated using Transwell assay. n, Quantification analysis of invasion ability of 786-O cells evaluated using Transwell assay. o. Apoptotic rate in 786-O cell analyzed using flow cytometry. p, PCNA, KI67, MMP2, MMP9, Cyclin D1, Cyclin E, Caspase-3, and Caspase-9 protein expression in 786-O cells measured using western blot analysis. ^*p < 0.05 vs. adjacent normal tissues or 786-O cells treated with oe-NC; # p < 0.05 vs. the 786-O cells treated with oe-CEP55. The data were measurement data and described as mean ± standard deviation. Paired t-test was used for data comparison between RCC tissues and adjacent normal tissues. Unpaired t-test was used for analysis of data between the other two groups. Data among groups were compared with one-way ANOVA, followed by Tukey’s post hoc test. The data at different time points among different groups were analyzed by the repeated-measures ANOVA and Bonferroni’s post hoc test. The cell experiment was repeated 3 times. miR-144, microRNA-144; CEP55, centrosomal protein 55; RT-qPCR, reverse transcription quantitative polymerase chain reaction; circRNA, circular RNA; mRNA, messenger RNA; CCK-8, cell counting kit-8