Figure 2.
Effect of alkyl methacrylate monomer chain length and composition and on pH-responsive responsive properties of diblock copolymers. (a) Particle diameter (number-average) and polydispersity (PDI) of mPEG-block-[DMAEMA-co-AnMA[%]] composed of alkyl methacrylate monomers of different chain length (n) and composition ([%]) in phosphate buffered saline (PBS) at pH 7.4. Data are mean ± SD with n = 3 experimental replicates. (b) Heat map demonstrating the fold-decrease in particle diameter between pH 7.4 and pH 5.8 for polymers of indicated n and [%]. (c) Fraction of red blood cell hemolysis mediated by polymers (1 μg/mL) at indicated pH values. Data are mean ± SD and representative of two independent experiments, with n = 4 biological replicates. Statistical analysis is provided in Tables S1 and S2 (Supporting Information). (d) Heat map summarizing hemolysis data in (c) at pH 7.4, pH 6.6, and pH 5.8.