Figure 3.

Identification of lead polymeric carriers for delivery of 3pRNA RIG-I agonists. (a) Effect of charge ratio (N:P) on degree of electrostatic complexation of dsRNA by polymeric carriers. (b) Representative dose response curve in A549 ISG reporter cells using polymers containing variable amounts of BMA (n = 4) to deliver 3pRNA. (c) Summary of approximated half-maximal effective concentration (EC₅₀) of 3pRNA delivered with polymeric carriers. For graphical representation, a value of 1000 nM is assigned to all carriers for which an EC₅₀ could not be estimated due to insufficient activity in the dose range explored. (d) Heat map depicting the ratio of the minimum estimated EC₅₀ (4–50) to the EC₅₀ of the indicated polymer. All values plotted as mean ± SD.