Figure 4.
Effect of alkyl monomer chain length and composition on endosomal escape. (a) Schematic of Galectin 8 (Gal8) recruitment assay used to investigate endosomal escape of selected carriers. Image created with BioRender.com. (b) Mean number of YFP-Gal8 vesicles observed per cell for indicated polymers loaded with control dsRNA. (c) Representative fluorescent images of cells expressing Gal8-YFP fusion protein upon treatment with 12–60 (low degree of endosomal escape), 8–40 (medium escape), 10–40 (high escape), or untreated (NT). (d) Dose response curves of relative luminescence upon treatment of luciferase-expressing MDA-MB-231 with indicated carrier complexed with luciferase siRNA. (e) Summary of estimated half-maximal effective concentration values (EC50) of the data shown in (e). All values are plotted as mean ± SD **P < 0.01, ****P < 0.0001 by one-way ANOVA with Tukey’s posthoc test.