In vivo evaluation
of lead 3pRNA carriers. (a) Schematic of in vivo analysis
of 3pRNA carrier activity in mice. Image
created with BioRender.com.
(b) Serum levels of IFN-α following intravenous administration
of 3pRNA using indicated carrier. qRT-PCR analysis of (c) Ifnb1 and (d) Cxcl10 expression in liver,
spleen, lungs, and kidney of mice following intravenous administration
of 3pRNA using indicated carrier. Vehicle (veh) is PBS. All values
plotted as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs vehicle by one-way ANOVA with Dunnett’s
multiple comparison test.