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. 2020 Oct 26;5(11):3520–3532. doi: 10.1021/acssensors.0c01641

Table 2. Comparison of Existing Techniques for the Anti-PLA2R Detection.

    detection limit
       
technique label lower (ng/mL) upper (ng/mL) sample assay time (hr) cost (per instrument or test kit) ref.
commercial ELISA yes 0.6 10 buffer or diluted serum 1:100 3.5 inst.: £50k (55)
test: £30
fluoroimmunoassay yes 30 3400 buffer or diluted serum 1:100 3.5 inst.: £50k (34)
test: £300
SPR no 100 >3000 buffer 1 inst.: £200k (36)
test: £400
QCM-D (QSense, 5 MHz crystal) no 500 105 (100 μg/mL) buffer or concentrated serum 1 inst.: £40–£100k this work,56
test kit: £200–£400
C-QCM (10 MHz crystal) no 100 105 (100 μg/mL) buffer or concentrated serum 0.5 inst.: £1000 this work
test kit: £20