Table 2. Comparison of Existing Techniques for the Anti-PLA2R Detection.
detection
limit |
|||||||
---|---|---|---|---|---|---|---|
technique | label | lower (ng/mL) | upper (ng/mL) | sample | assay time (hr) | cost (per instrument or test kit) | ref. |
commercial ELISA | yes | 0.6 | 10 | buffer or diluted serum 1:100 | 3.5 | inst.: £50k | (55) |
test: £30 | |||||||
fluoroimmunoassay | yes | 30 | 3400 | buffer or diluted serum 1:100 | 3.5 | inst.: £50k | (34) |
test: £300 | |||||||
SPR | no | 100 | >3000 | buffer | 1 | inst.: £200k | (36) |
test: £400 | |||||||
QCM-D (QSense, 5 MHz crystal) | no | 500 | 105 (100 μg/mL) | buffer or concentrated serum | 1 | inst.: £40–£100k | this work,56 |
test kit: £200–£400 | |||||||
C-QCM (10 MHz crystal) | no | 100 | 105 (100 μg/mL) | buffer or concentrated serum | 0.5 | inst.: £1000 | this work |
test kit: £20 |