Fig. 5.

mTORC1 regulates the expression of CASP1 and GSDMD through STAT3 in macrophages. Macrophages were treated with rapamycin (100 nM) for 6 h, and the activity of mTORC1 and STAT3 and the expression of pyroptosic genes were examined. a Western blot analysis of mTORC1 activation and STAT3 activation. Phosphorylation of STAT3 was inhibited. b Expression of CASP1 and GSDMD, mRNA upregulated by rapamycin. The resolved bands were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA). *p < 0.05; **p < 0.01. n = 3 independent experiments. Error bar indicates SD