FIG. 5.

ABTB1 regulates hepatocytes proliferation by FOXG1/p21 pathway in vitro and in vivo. (A) The heat map shows top differentially up‐ or down‐regulated genes obtained from microarray data in siAbtb1‐transfected primary mouse hepatocytes. (B) Microarray‐based mRNA expression analysis shows knockdown of Abtb1 expression and up‐regulation of Foxg1 expression. (C) Gene ontology analysis of differentially expressed genes identified in siAbtb1‐transfected hepatocytes and their P values obtained using DAVID 6.8. (D) KEGG pathway enrichment and statistical analysis of differentially expressed genes identified in siAbtb1‐transfected hepatocytes. (E) Western blot analysis of FOXG1 and P21 in siAbtb1‐transfected hepatocytes and their quantification. Vinculin was used as a loading control. (F) qRT‐PCR analysis of Foxg1 expression at the indicated time points during the termination phase. (G) Schematic representation of the experimental design. (H) qRT‐PCR analysis of Foxg1 expression at day 5 after 2/3 PH in mice livers injected with AAV8‐Ttr‐miR‐125b‐5p. (I) Western blot analysis of FOXG1 and P21 at day 5 after 2/3 PH in mice livers injected with AAV8‐Ttr‐miR‐125b‐5p and their quantification. Vinculin was used as a loading control. (J) Schematic of miR‐125b‐5p‐mediated effect on hepatocyte proliferation through the ABTB1/FOXG1/p21 pathway. *P < 0.05, **P < 0.01; data are presented as mean ± SEM. Abbreviations: Crct1, cysteine rich C‐terminal 1; d, days; h, hours; Hrg, histidine rich glycoprotein; HTLV, human T‐lymphotropic virus; KEGG, Kyoto Encyclopedia of Genes and Genomes; MAPK, mitogen‐activated protein kinase; Proz, pritein Z; Thbs1, thrombospondin 1.