Figure 1.

Overview of the phenotyping pipeline. The plants are grown in 24-well trays in a greenhouse. At the appropriate stage, leaf fragments are harvested and mounted on standard 4-well microtiter plates, filled with 1% water agar for keeping the humidity, and inoculated by air-blowing of powdery mildew spores in an inoculation tower. After incubation of 5-7 days, the disease symptoms become visible. The plates with the infected leaves are loaded into the Macrobot system for automated imaging. The acquired images are transferred to the image analysis server for quantification of the disease symptoms.