Fig. 2.
8-Color CSF lymphoma panel using RPMI 1640. R1 (red) = CD19+ B lymphocyte population, R2 (green) = CD3+ T lymphocyte population. Top row plots (A,B): ungated data showing analysis gates. Plot (C), Forward vs. Side-scatter plot shows T cells as small to intermediate in size and B cells intermediate to large in size. Middle row plots (D–F): CD19+B cells are positive for Lambda light chain and negative for Kappa light chain; bottom row plots (G–J): CD3+ T cells are positive for CD8 and CD7, and there are separate populations of T cells that are CD8+CD4− and CD8−CD4+.
Summary of analysis: R1-gated population expresses pan B-cell marker CD19 and co-expresses Lambda light chain with little or no expression of Kappa light chain. T cell markers CD3 and CD7 appear normal as do T cell subsets CD4 and CD8. Patient previously diagnosed with follicular center-cell lymphoma confirmed by flow studies of peripheral blood. CNS involvement confirmed with identification of same phenotypic population in CSF specimen submitted for flow cytometry analysis.
- Kappa FITC (Becton-Dickinson, clone TB28-2, cat. no. 643774)
- Lambda PE (Becton-Dickinson, clone 1-155-2, cat. no. 642924)
- CD4 Pac Blue (Becton-Dickinson, clone SK3, cat. no. 558116)
- CD19 PC7 (Beckman Coulter, clone J3-119, cat. no. IM3628U)
- CD8 APC, (Becton-Dickinson, clone SK1, cat. no. 340659)
- CD3 APC-H7, (BD, clone SK7, cat. no. 641406)
- CD7 PerCP-cy5.5, (Becton-Dickinson, clone MT701, cat. no. 561602