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(A) Knock-out recombination strategy. (B) Southern blotting of wild-type (+/+) and homozygous deleted (−/−) Kai1/Cd82 mice. (C) Kai1/Cd82 immunoblot in proteins isolated from wild-type (+/+) and homozygous deleted (−/−) Kai1/Cd82 mouse spleens. (D) Immunohistochemical detection of Kai1/Cd82 in kidney sections from wild-type (+/+), heterozygous (+/−), and knockout (−/−) mice.
