Example of how the RhizoVision Analyzer extracts quantitative features from the root crown image. For each pixel within the root crown skeleton, the corresponding value from the distance map is used to estimate root diameter (a). Topological information is extracted from the skeletal structure such as branch points (shown in blue), root direction change (shown in orange), and end points (shown in green) (b). Finally, for the root counting procedure (c), a pixel transition is marked in a horizontal line scanning operation (shown in blue) for each row and is recorded for counting the number of roots in that row (shown in red). This profile is then sorted to extract the median and maximum number of roots.