Figure 6.

IR/PDK1 involved mechanism in the protective effects of hesperidin. At the termination of animal study, fat tissues were collected for western blot analysis. GAPDH was used as the loading control. All expression data were normalized to GAPDH. (A) Representative blot. Statistical analysis of (B) IR and (C) PDK1 expression levels. Data are presented as the mean ± SD. Experiments were repeated ≥3 times. ^**P<0.01 vs. vehicle group. IR, insulin receptor; PDK1, phosphoinositide-dependent kinase 1; p-, phosphorylated.