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. 2020 Nov 19;21(1):52. doi: 10.3892/etm.2020.9484

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Klotho, Nrf 2, ARE and caspase-9 protein and mRNA expression levels of different groups determined by WB and RT-PCR assay. (A) Relative protein expression of different groups by WB assay. (B) Relative mRNA expression of different groups by RT-PCR assay. Sham, sham group; model, myocardial infarction model group; pDC316, empty pDC316 were injected in rats based on myocardial infarction model group; LY294002, injection with LY294002 based on myocardial infarction model group; pDC316-Klotho, Klotho gene with pDC316 were injected in rats based on myocardial infarction model group. ^***P<0.001 vs. sham group; ^##P<0.01 vs. model group. WB, western blotting; RT-PCR, reverse transcription-PCR.