Table 3.

Reproducibility (Coefficient of Variation, %) of the three qRT-PCR assays.

Assay			Copy number of RNA transcript
			1 × 106	1 × 105	1 × 104	1 × 103	1 × 102	1 × 101
N gene assay	IPBCAMS assays	Intra-assay	0.52*	1.33	0.37	0.46	0.20	1.25
		Inter-assay	1.06	2.45	1.49	1.32	1.37	1.45
	WHO assays	Intra-assay	1.08	1.19	1.12	0.87	0.46	5.09
		Inter-assay	7.59	2.94	2.78	6.60	0.96	3.77
	CCDC assays	Intra-assay	0.52	0.54	0.27	0.74	0.41	1.97
		Inter-assay	1.56	1.20	5.51	1.00	1.40	2.89
ORF 1b gene assay	IPBCAMS assays	Intra-assay	0.73	0.26	1.10	1.30	4.45	3.36
		Inter-assay	4.66	3.85	2.77	2.17	5.12	3.50
	WHO assays	Intra-assay	0.57	0.47	0.88	0.41	0.29	1.76
		Inter-assay	1.57	0.30	0.87	0.69	0.55	1.23
	CCDC assays	Intra-assay	1.66	0.78	0.71	0.92	2.45	6.52
		Inter-assay	0.52	0.54	0.27	0.74	0.41	1.97

The coefficient of variation was calculated by the standard deviation of the Ct values of an RNA dilution divided by the mean Ct values of the corresponding RNA dilution.