Table 4.
Evaluation of the three qRT-PCR assays with clinical specimens.
| Specimen ID | Specimen type | N gene assay |
ORF 1b gene assay |
||||
|---|---|---|---|---|---|---|---|
| IPBCAMS | WHO | CCDC | IPBCAMS | WHO | CCDC | ||
| TS98 | Throat swab | 35.79 | NA | 35.42 | NA | NA | NA |
| TS101 | Throat swab | 33.48 | NA | 34.24 | NA | NA | NA |
| TS103 | Throat swab | NA | NA | 34.68 | NA | NA | NA |
| TS105 | Throat swab | 31.5 | 35.76 | 31.64 | NA | NA | NA |
| TS108 | Throat swab | 33.35 | NA | 32.11 | 33.36 | NA | NA |
| TS110 | Throat swab | 29.99 | 31.73 | 29.1 | 33.57 | NA | NA |
| TS165 | Throat swab | 27.34 | 30.46 | 28.14 | 31.06 | 27.84 | NA |
| TS168 | Throat swab | NA | NA | 34.97 | NA | NA | NA |
| TS169 | Throat swab | 33.34 | NA | 34.04 | NA | 34.2 | NA |
| TS187 | Throat swab | 34.5 | 39.2 | 33.03 | NA | NA | NA |
| TS188 | Throat swab | 35.03 | 35.9 | 33.57 | NA | 24.07 | NA |
| TS189 | Throat swab | 31.16 | 35.43 | 31.21 | 34.04 | 30.92 | NA |
| TS190 | Throat swab | 32.84 | 34.02 | 32.56 | NA | NA | NA |
| TY1 | Sputum | 27.35 | 29.44 | 27.6 | 30.98 | 27.33 | NA |
| TY2 | Sputum | 29.38 | 31.26 | 29.06 | 32.32 | 28.72 | NA |
| TY3 | Sputum | 31.85 | NA | 31.3 | 35.84 | NA | NA |
| TY4 | Sputum | 22.99 | 25.57 | 22.08 | 27.42 | 24.12 | 35.99 |
| TY6 | Sputum | 25.51 | 27.52 | 25.58 | 29.03 | 25.58 | 41.54 |
| TY7 | Sputum | 26.9 | 30.21 | 27.4 | 30.05 | 27.3 | 45.26 |
| TY8 | Sputum | 29.21 | 31.87 | 30.06 | 33.65 | 29.84 | NA |
| TY9 | Sputum | 26.29 | 28.45 | 26.34 | 30.69 | 26.03 | 46.34 |
| XT1 | Sputum | 25.74 | 27.26 | 25.3 | 29.82 | 26.34 | 45.9 |
| XT2 | Sputum | 31.57 | NA | 30.95 | 34.19 | NA | NA |
| XT3 | Sputum | 31.14 | NA | 32.02 | 35.02 | NA | NA |
| XT4 | Sputum | 32.67 | NA | 31.71 | 34.26 | NA | NA |
| account (%) of positive | 23 (92 %) | 15 (60 %) | 25 (100 %) | 16 (64 %) | 12 (48 %) | 5(20 %) | |