Increased migrasome release during podocyte injury. HPCs were treated with LPS, PAN, or HG at different concentration or for different times. The number of migrasomes was detected and analyzed by NanoSight. a HPCs were treated with LPS at 50 μg/mL for different times (left panel) or various LPS concentration for 6 h (right panel). b HPCs were treated with PAN at 75 μg/mL for different times (left panel) or various concentration of PAN for 24 h (right panel). c HPCs were treated with HG at 60 mM for different times (left panel) or different concentration of glucose for 48 h (right panel). Data represent mean ± SEM, and p value was analyzed by 2-tailed Student's t test. **p < 0.01, ***p < 0.001, ****p < 0.0001. HPCs, human podocyte cell lines; PAN, puromycin amino nucleoside.