Figure 4.

Temporal profiles of core clock and proliferation regulating genes in residual β cells and adjacent α cells following massive ablation. (A) Phase shift between rhythmically expressed transcripts in CTR and DOX conditions (model CC) identified in α cells (in green) and β cells (in red) according to the temporal analysis of RNA-seq presented in Figure 3. Phase differences were calculated for each rhythmic transcript and plotted on the graph. 0 corresponds to identical phase between the transcript expression in CTR and DOX conditions. (B) Temporal profiles of selected core clock transcripts (RorC, Arntl [Bmal1], and Cry1) in control α and β cells (in green and in red, respectively; left panels), in control β cells versus residual counterparts (middle panels), and in control and DOX-treated α cells (right panels) assessed by RNA-seq. (C) Temporal profiles of selected transcripts encoding for the proliferation regulating proteins (see also Fig. 3G) in sorted β cells. Data are expressed as mean ± SD for two experimental repetitions per each time point with at least three mice in each plicate.