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. 2020 Dec 1;34(23-24):1697–1712. doi: 10.1101/gad.340604.120

Figure 5.

Figure 5.

DUSP11 promotes RNA virus replication by dephosphorylating viral RNA PAMPs. (A) Schematic diagram of the VSV leader and trailer RNA 5′ end characterization assay. RNA was extracted from A549 or HEK293 WT and DUSP11 KO cells infected with VSV at MOI of five PFU/cell for 24 h and treated with or without 5′-monophosphate-dependent exonuclease XRN1. Purified RNA was then subject to Northern blot analysis. (B) Northern blot analysis on VSV leader, trailer and small RNAs purified from A549 WT/DUSP11 KO cells infected with WT VSV. (C) Northern blot analysis on VSV leader, trailer, and small RNAs purified from HEK293 WT/DUSP11 KO cells infected with WT VSV. (D) Graphical representation of the relative band density percentage ratio (+XRN1/−XRN1) of VSV leader and trailer RNA in A549 WT and DUSP11 KO cells as determined by Northern blot analysis in B. (E) Graphical representation of the relative band density percentage ratio (+XRN1/−XRN1) of VSV leader and trailer RNA in HEK293 WT and DUSP11 KO cells as determined by Northern blot analysis in C. (F) Graphical representation of the relative band density percentage ratio (+XRN1/−XRN1) of 5.8s rRNA in A549 WT and DUSP11 KO cells as determined by Northern blot analysis in B. Data are derived from n = 4 independent replicates for D and F and n = 3 independent replicates for E. In all panels, data are presented as mean ± SEM.