Table 1. Primers and probe sequences used for in-house diagnostic assays used to test persons with suspected SARS-CoV-2 infection after flight from Sydney to Perth, Australia, March 19, 2020.
Oligonucleotide |
Sequence, 5¢®3¢ |
Concentration, μM |
---|---|---|
BetaCoV_Wuhan_F22595 (forward) | TGAAGTTTTTAACGCCACCAGAT | 0.7 |
BetaCoV_Wuhan_R22662 (reverse) | CACAGTTGCTGATTCTCTTCCTGTT | 0.9 |
BetaCoV_Wuhan_P22619-HEX (hydrolysis probe) | (HEX)C[+A][+A]GCAT[+A][+A]A[+C]AGATGCA(BHQ1) | 0.3 |
MS2-105F (forward) | GTCGACAATGGCGGAACTG | 0.2 |
MS2-170R (reverse) | TTCAGCGACCCCGTTAGC | 0.5 |
MS2-127-VIC (hydrolysis probe) | (VIC)ACGTGACTGTCGCCCCAAGCAACTT(QSY) | 0.2 |