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. 2020 Dec;26(12):2872–2880. doi: 10.3201/eid2612.203910

Table 1. Primers and probe sequences used for in-house diagnostic assays used to test persons with suspected SARS-CoV-2 infection after flight from Sydney to Perth, Australia, March 19, 2020.

Oligonucleotide
Sequence, 5¢®3¢
Concentration, μM
BetaCoV_Wuhan_F22595 (forward) TGAAGTTTTTAACGCCACCAGAT 0.7
BetaCoV_Wuhan_R22662 (reverse) CACAGTTGCTGATTCTCTTCCTGTT 0.9
BetaCoV_Wuhan_P22619-HEX (hydrolysis probe) (HEX)C[+A][+A]GCAT[+A][+A]A[+C]AGATGCA(BHQ1) 0.3
MS2-105F (forward) GTCGACAATGGCGGAACTG 0.2
MS2-170R (reverse) TTCAGCGACCCCGTTAGC 0.5
MS2-127-VIC (hydrolysis probe) (VIC)ACGTGACTGTCGCCCCAAGCAACTT(QSY) 0.2