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. 2020 Dec;26(12):2970–2973. doi: 10.3201/eid2612.203309

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Elution of SARS-CoV-2 anti-spike glycoprotein antibodies from DBS samples, showing 3-fold DBS eluate (A) (initial 1:3 dilution) and serum (B) (initial 1:15 dilution) titrations. Dashed line indicates pre–August 2019 DBS samples (n = 11). Red circles indicate PCR-positive samples (n = 5). Black circles indicate PCR-unknown samples (n = 11), from matched contemporaneous samples. All samples were selected at random for inclusion. DBS, dried blood spot; OD₄₅₀, optical density at 450 nm; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.