TABLE 1.
List of primers and TaqMan assay probes used for PCRs (qRT‐PCR and genomic DNA PCR)
| Gene | Forward primer (5′‐3′) | Backward primer (5′‐3′) |
|---|---|---|
| Rpl32 | GCTGGAGGTGCTGCTGATGT | ACTCTGATGGCCAGCTGTGC |
| Bdnf | GGACTCTGGAGAGCGTGAAT | GTCCTCATCCAGCAGCTCTT |
| Tnf‐α | GACGTGGAACTGGCAGAAGA | GCCACAAGCAGGAATGAGAA |
| Il6 | CCGGAGAGGAGACTTCACAG | TCCACGATTTCCCAGAGAAC |
| Cox‐2 | CTCCCTGAAGCCGTACACAT | CCCAAAGATAGCATCTGGA |
| HIV‐1 LTR | GGTCTCTCTGGTTAGACCAGAT | CTGCTAGAGATTTTCCACACTG |
| HIV‐1 nef | ATGGGTGGCAAGTGGTCAA | TCAGCAGTTCTTGAAGTACTC |
| HIV‐1 tat | GGAATTCACCATGGAGCCAGTAGATCCT | CGGGATCCCTATTCCTTCGGGCCTGT |
| 18s | CTTAGAGGGACAAGTGGCG | ACGCTGAGCCAGTCAGTGTA |
| Gapdh | CATCCTGCACCACCAACTGCTTAG | GCCTGCTTCACCACCTTCTTGATG |
| Gene | Company | TaqMan Assay Probe ID |
|---|---|---|
| Ngf | Applied Biosystems | Mm00443039_m1 |
| Ntf‐3 | Applied Biosystems | Mm01182924_m1 |
| Ntf‐4/5 | Applied Biosystems | Mm01701591_m1 |
| Rps18 | Applied Biosystems | Mm02601777_g1 |