FIGURE 5.

Triple immunofluorescence labelling for IBA‐1, GFAP, and CGRP in the lumbar 4–5 spinal dorsal horns (SDHs) of chronic constriction injury (CCI) mice pre‐administered activin C (250 ng in 200 μl of PBS) or PBS (200 μl) on day 7 after CCI. (a) Immunofluorescence images. (b–d) Quantitative analysis for the number of IBA‐1‐positive microglia (b), the density of GFAP immunoreactivity (c), and the density ratio of CGRP immunoreactivity (d).n = 6 mice; Cont., contralateral; Ipsi., ipsilateral; IBA‐1, ionized calcium‐binding adaptor molecule 1 (Iba1); GFAP, glial fibrillary acidic protein (GFAP). Data are presented as mean ± SEM. *P < 0.05, significantly different as indicated; one‐way repeated‐measures ANOVA followed by post hoc Bonferroni multiple comparisons test for (b) and (c) and unpaired two‐tailedt‐test with Welch's correction for (d)