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. 2020 Nov 9;177(24):5595–5608. doi: 10.1111/bph.15270

TABLE 1.

Sequences of the primers used for RT‐PCR experiments of the tracheal epithelium

nAChR subunit Accession no. Sequence Product length (bp)
α2 NM_144803 fwd: ctcccatcctgctttccag 115
rev: gtttgaacaggcggtcctc
α3 NM_145129 fwd: cgcctgttccagtacctgtt 196
rev: cagagggtttccatttcagc
α4 NM_015730 fwd: ctcagatgtggtccttgtcc 238
rev: ggtgggtgactgcaaagttc
α5 NM_176844 fwd: ccagctaatgaccaccaacg 218
rev: gctgcgtccaagtgacagt
α6 NM_021369 fwd: cctgcactgcggtttatgtc 231
rev: cagccacagattggtctcca
α7 NM_007390 fwd: acaatacttcgccagcacca 144
rev: aaaccatgcacaccagttca
α9 NM_001081104 fwd: caatgctctgcgtccagtag 208
rev: acaccagatcgctgggaa
α10 NM_001081424 fwd: tctgctcctgctctttctcc 207
rev: ccacaggtacaaggtcagca