Figure 4.

Monocytes Dramatically Reduce Expression of MHC Class II Proteins Independent of Infection Status

(A) Expression of major histocompatibility (MHC) or MHC-associated genes (rows) in key cell types at baseline (B), early (E), middle (M), or late (L) EVD (columns). Circle size: percentage of cells in that group in which the gene was detected; color: mean expression in Z score normalized, log_e transcripts per 10,000 (TP10K). The “MAMU-” prefix, which designates MHC genes in rhesus monkeys, was removed; the “HLA-” prefix is indicated by “(H).”

(B) CyTOF intensity of HLA-DR protein in antigen-presenting cells. Boxes: median and interquartile range; whiskers: 2.5^th and 97.5^th percentiles. Colored stars indicate significant decreases from baseline (rank-sum test p < 0.05) with color corresponding to stage.

(C and D) Fold change (log₂ scale) in average CD38 (C) and HLA-DR (D) CyTOF intensity on monocytes at each DPI relative to baseline, connected by colored lines for each NHP. See also Figure S4C and Data S1.

(E) Average gene expression (log_e TP10K) for four MHC class II genes in monocytes, stratified by cell-infection status. Error bars: 95% CI on the mean based on 200 bootstraps.