KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| cyclin D1 | Santa Cruz | sc-20044; RRID: AB_627346 |
| Akt1 | Santa Cruz | sc-5298; RRID: AB_626658 |
| pAkt1 Ser473 | Cell Signaling | #9018; RRID: AB_2629283 |
| pAkt1/2/3 Ser473 | Santa Cruz | sc-7985-R; RRID: AB_667741 |
| TSC2 | Santa Cruz | sc-893; RRID: AB_632569 |
| pTSC2 Ser939 | Abcam | ab59269; RRID: AB_2210004 |
| FKHR | Santa Cruz | sc-11350; RRID: AB_640607 |
| pFKHR Ser319 | Santa Cruz | sc-101682; RRID: AB_2294254 |
| Bad | Santa Cruz | sc-8044; RRID: AB_626717 |
| pBad Ser136 | Abcam | ab28824; RRID: AB_725616 |
| Rictor | Santa Cruz | sc-271081; RRID: AB_10611167 |
| Rictor | Santa Cruz | sc-99004; RRID: AB_2269610 |
| pRb Ser780 | Cell signaling | #9307; RRID: AB_330015 |
| Flag (M2) | Millipore-Sigma | F1804; RRID: AB_262044 |
| HA | Santa Cruz | sc-805; RRID: AB_631618 |
| cyclin A2 | Abcam | ab16726; RRID: AB_302478 |
| PACSIN 2 | Proteintech | 10518–2-AP; RRID: AB_2161854 |
| Paxillin (5H11) | Millipore-Sigma (Upstate) | 05–417; RRID: AB_309724 |
| pPaxillin Tyr118 | Thermo Fisher | 44–722G; RRID: AB_2533733 |
| β-actin | Santa Cruz | sc-47778; RRID: AB_2714189 |
| Bacterial and Virus Strains | ||
| JM109 Competent Cells | Promega | L2001 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| AKT1 (NM_005163) Human Recombinant Protein | Origene | TP320257 |
| Insulin Human Recombinant Protein | Millipore-Sigma | I2643 |
| Recombinant human EGF protein, CF | R&G | 236-EG |
| Critical Commercial Assays | ||
| Duolink In Situ Red Starter Kit Mouse/Rabbit | Millipore-Sigma | DUO92101 |
| RNeasy Mini Kit | QIAGEN | 74104 |
| SuperScript™ III First-Strand Synthesis System | Thermo Fisher | 18080051 |
| Deposited Data | ||
| cyclin D1-dependent gene signature in mouse mammary gland | GEO database | GEO: GSE43216 |
| Akt1-dependent gene signature from the murine ErbB2 mammary tumor | GEO database | GEO: GSE138957 |
| Experimental Models: Cell Lines | ||
| MCF-7 | ATCC | HTB-22 |
| HEK293T | ATCC | CRL-3216 |
| Cyclin D1 wt and ko MEFs/3T3s | Casimiro et al., 2012 | N/A |
| ErbB2-Akt wt and ko mammary tumor cells | Ju et al., 2007 | N/A |
| Inducible rictor shRNA MEFs (iRicKO) | Cybulski et al., 2012 | N/A |
| Experimental Models: Organisms/Strains | ||
| MMTV-Rtta-control, cyclin D1-wt and cyclin D1-KE mice/FVB | Casimiro et al., 2012, 2015 | N/A |
| MMTV-ErbB2-Akt1 wt and ko mice/FVB | Ju et al., 2007 | N/A |
| Flox-cyclin D1/Rosa26-CreERT2 mice/FVB | Choi et al., 2012 | N/A |
| Recombinant DNA | ||
| p3xFlag-CMV-10 encoding cyclin D1 and its mutants | Wang et al., 2003 | N/A |
| pMSCV -IRES-GFP encoding cyclin D1 and its mutants | Li et al., 2006b | N/A |
| ECFP-Mem-Cyclin D1 plasmid | This paper | N/A |
| Cherry-lacR-NLS-CD1NUC Plasmid | Li et al., 2010 | N/A |
| pBABE-myrAkt1-IRES-GFP | Eves et al., 1998 | N/A |
| pCMV5-HA-Akt1 | Alessi et al., 1996 | N/A |
| pGEX-GST-Akt1 and pGEX-GST-Akt1(S473A) | Liu et al., 2014 | N/A |
| c-fos-LUC, SRE orTCF mutant (pm12 or pm18) | Wang et al., 1998 | N/A |
| pRSV-β-Gal | Addgene | #24058 |
| Software and Algorithms | ||
| Fiji (ImageJ) | ImageJ | https://imagej.net/Fiji |