Table 6.

Results of generalized linear regression models for the main effect of sub-stage and PFAA and interaction terms. The main effect beta value represents the change in PFAA with one increase sub-stage development. The p-values determine whether the associated beta value represents a significant or non-significant change in PFAA with progressing egg development (sub-stage). Interaction term beta values determine the estimated change in PFAA by sub-stage based on the length or weight of the fish and can be used to calculate the change in various length or weight fish for each PFAA. Interaction term p-values determine if total length (Model 1) or Fish Weight (Model 2) beta values significantly affect the relationship between PFAA and sub-stage of development. Non-significant interaction terms were not included in the table.

PFAA	Model 1. Substage, total length, and substage * length interactiona		Model 2. Substage, fish weight, and substage * weight interaction
	Substage main effect	Substage * length interaction	Substage main effect	Substage * weight interaction
	Beta and p-value	Beta and p-value	Beta and p-value	Beta and p-value
PFOA	−0.3895 (0.09)	0.0113 (0.03)	−0.0758 (0.29)	0.0002 (0.02)
PFNA	−2.2263 (<0.01)	0.0563 (<0.01)	−0.6541 (< 0.01)	0.0010 (<0.01)
PFDA	−0.10952 (0.44)	N/A	−0.1074 (0.45)	N/A
PFUnA	−0.0238 (0.88)	N/A	0.07235 (0.65)	N/A
PFDoA	0.9163 (0.06)	−0.0199 (0.08)	0.3856 (0.01)	−0.0003 (0.07)
PFTriA	−0.2940 (0.053)	0.0078 (0.03)	−0.0499 (0.30)	0.0001 (0.03)
PFOSA	0.8755 (<0.01)	−0.0238 (<0.01)	0.2089 (0.01)	−0.0004 (<0.01)
PFOS	−56.817 (0.04)	N/A	−56.799 (0.04)	N/A
PFHxS	−1.5324 (0.16)	N/A	−1.6832 (0.12)	N/A

^aWe present model results with the interaction term if it was significant at p < 0.10. If not, we present the model results controlling for total length or fish weight, but not including the interaction term.