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Outer retina was selected as the region of interest, corresponding to total positive ion MALDI mass spectra (A). Extracted positive ion MALDI images of the [M+Na]+ of PC44:12 (m/z 900.6 amu, total photoreceptor layer) (B), and of the [M+H]+ of PC54:12 (m/z 1018.7 amu, photoreceptor outer segments) (C). D: Merged positive ion MALDI images of PC54:12 (red) and PC44:12 (green).
