Fig. 2.

StarD1 overexpression in B6/129 mice led to hepatic Cyp7b1 mRNA downregulation without altering gallbladder total bile acid concentration. A: Effect of Ad-StarD1 overexpression on hepatic Cyp7b1 and Cyp27a1 mRNA expression. B: Gallbladder bile acid concentration (a) and composition (b) of StarD1-overexpressed B6/129 male mice. Bile acids were measured by HPLC after deconjugation (see Methods). Each bile acid was the sum of unconjugated, 24-aminoacyl conjugated, 3-sulfated, 3-glucuronidated, and double-conjugated forms of bile acids. i.e., CAs represents the total of CA, TCA, GCA, CA3S, CA3G, TCA3S, and GCA3S. MCAs include three isomers (α-, β-, and ω-MCA) and their conjugated forms. Abbreviations are shown in supplemental Fig. S1 (n = 4 in each group). Welch’s t-tests were performed between β-Gal control and StarD1-overexpressed mice and significance indicated by ***P ≤ 0.001.