Fig. 3.

Sterile inflammation is associated with increased oxysterol formation in mice. StarD1 was overexpressed in B6/129 male mice to drive endogenous oxysterol formation as outlined in the Methods. Mouse livers were evaluated at day 14, which was 7 days following StarD1 overexpression, by both flow cytometry and qPCR. All cells were gated off of live singlets. A: Measurement of total percent hematopoietic cells (CD45⁺), percent B cells (CD45⁺B220⁺MHCII⁺), percent eosinophils (CD45⁺CD11b⁺CCR3⁺), percent neutrophils (CD45⁺CD11b^hiF4/80^lowLy6G⁺Ly6C⁺), percent dendritic cells (CD45⁺CD11c⁺MHCII⁺), and percent Kupffer cells (CD45⁺CD11b⁺F4/80⁺). B: Normalized relative mRNA expression of inflammation markers related to NAFLD, normalized to the housekeeping gene (Tbp). Statistics were performed as a Student’s t-test between groups in A and a Mann-Whitney nonparametric comparison in B. Error bars depict SEM. *P ≤ 0.05 versus β-Gal control.