Effect of WD feeding (2–6 weeks) on key hepatic oxysterols, Cyp7b1 and Ch25h mRNA expression, and histological inflammation in B6/129 mice. A: Representative liver histology (H&E staining, ×40) of RC-fed control and timed WD-fed B6/129 mice. B: Relative hepatic Cyp7b1 and Ch25h mRNA expression levels of B6/129 mice fed WD. C: Hepatic key oxysterol and 3β-HCA levels of WD-fed B6/129 mice. All mice were 12 weeks old when euthanized except for the 6 week WD-fed group (marked as “†”), which was 15 weeks old when euthanized. The 12-week-old B6/129 mice fed with RC were used as controls. RC, irradiated Teklad LM-485; WD, Harlan-Teklad TD.88137. RC control, n = 5; 2 Week WD, n = 5; 4 Week WD, n = 4; 6 Week WD, n = 3. Welch’s t-tests were performed between RC-fed animals and each group of WD-fed animals. Significance is indicated by *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.