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. 2020 Oct 2;61(12):1629–1644. doi: 10.1194/jlr.RA120000924

Fig. 5.

Fig. 5.

Hepatic Cyp7b1 and Ch25h mRNA expression in the insulin-resistant mouse models: The 2-day-old male pups of C57Bl/6J mice were given low dose (200 μg/mouse) STZ to partially destroy β cells as described in the Methods. The mice were fed a low-fat RC diet (CE-2, CLEA Japan Inc.) until 4 weeks of age (STZ-RC), and then fed an ad libitum HFD (HFD32, CLEA Japan Inc.) until age 8 weeks (STZ-HFD, STAM™). One group of mice was continued on their RC diet for an additional 4 weeks (8-week-old STZ-RC). Controls (WT-RC) were 4- or 8-week-old untreated C57Bl/6J mice fed RC. STZ-RC (4 weeks old, n = 5; 8 weeks old, n = 6), STZ-HFD (8 weeks old, STAM™, n = 5), WT-RC (4 weeks old, n = 5; 8 weeks old, n = 6). Welch’s t-tests were performed between WT and STZ-treated animals of the same age. Significance is indicated by *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.