Fig. 5.

Overexpression of SCD1 promotes fat mobilization and thermogenesis in scWAT and enhances whole-body energy expenditure of mice. A: Body weight change of mice during four adenovirus injections (n = 6 for each group). B: Total food consumption of mice injected with adenovirus for 2 weeks (n = 6 in each group). C: Representative Western blots from scWAT, gWAT, and BAT adipocytes of SCD1. D: H&E staining of scWAT from mice injected with Ad-LacZ or Ad-SCD1 adjacently to the scWAT. E: Representative Western blots from scWAT adipocytes of ATGL and HSL. The same samples from the same mouse scWAT in C and E. F: Basal OCR of scWAT was measured and shown (n = 6 in each group). Data were normalized to the Ad-LacZ group. G: Relative mRNA expression of the transcriptional regulators, FA oxidation enzymes, and nuclear-encoded mitochondrial genes in scWAT treated with Ad-LacZ or Ad-SCD1 (n = 4 in each group). H: Whole-body oxygen consumption rate (VO₂) of the mice during a 12 h light/12 h dark cycle was measured and the mean of the 12 h light/12 h dark cycle were displayed (n = 8 mice in each group). I: Heat generation of mice in a 12 h light/12 h dark cycle was calculated and the average values for the 12 h light/12 h dark cycle are displayed (n = 8 mice in each group). J: Rectal temperature of mice injected with Ad-LacZ or Ad-SCD1 at 4°C for 7 h (n = 6 for each group). Statistical analysis: unpaired Student’s t-test in A, B, D, F, and G; two-way ANOVA in H, I, and J. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.