Fig. 6.

In mice, SCD1 knockdown inhibits fat mobilization in scWAT lipolysis and decreases whole-body energy expenditure. A: Body weight change of mice during four adenovirus injections (n = 6 for each group). B: Total food consumption of mice injected with adenovirus for 2 weeks (n = 6 in each group). C: Representative Western blots from scWAT, gWAT, and BAT adipocytes of SCD1. D: H&E staining of scWAT from mice injected with Ad-shLacZ or Ad-shSCD1 adjacently to the scWAT. E: Representative Western blots from scWAT adipocytes of ATGL and HSL. The same samples from the same mouse scWAT in C and E. F: Whole-body oxygen consumption rate (VO₂) of the mice in basal and CL-316,243 conditions during a 12 h light/12 h dark cycle was measured and the mean in basal and CL-316,243 conditions of the 12 h light/12 h dark cycle were displayed (n = 8 mice in each group). G: Heat generation of mice in basal and CL-316,243 conditions in a 12 h light/12 h dark cycle was calculated and the average values for the 12 h light/12 h dark cycle in basal and CL-316,243 conditions are displayed (n = 8 mice in each group). H: Rectal temperature of mice injected with Ad-shLacZ or Ad-shSCD1 at 4°C for 7 h (n = 6 for each group). Statistical analysis: unpaired Student’s t-test in A, B, and D; two-way ANOVA in F, G. and H. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.