TABLE 5.

GO term enrichment analysis (by BP) applied to genes detected in both strains, CBS.FLAG.SRE1N and CBS.cyp61⁻.FLAG.SRE1, associated with ChIP-exo peaks

GO ID	BP and Associated Genes	Annotated	Significant	Expected	Rank in Classic Fisher	P (classic)	P (weight01)
GO:0006696	Ergosterol biosynthetic process	14	6	0.08	1	2.7E-11	2.7E-11
	g1377: HMGR
	g3385: SE-domain-containing protein (Squalene epoxidase)
	g3516: HMGS
	g5928: Cytochrome P450 oxidoreductase (CrtR)
	g904: Delta-sterol C-methyltransferase
	g989: C-22 sterol desaturase (Cyp61)
GO:0008299	Isoprenoid biosynthetic process	13	3	0.07	21	3.6E-05	6.7E-04
	g1377: HMGR
	g3516: HMGS
	g5104: Terpenoid synthase (CrtE)
GO:0008610	Lipid biosynthetic process	92	9	0.49	13	2.3E-10	2.3E-03
	g1377: HMGR
	g3385: SE-domain-containing protein (Squalene epoxidase)
	g3516: HMGS
	g5104: Terpenoid synthase (CrtE)
	g5794: Fatty acid hydroxylase
	g5928: Cytochrome P450 oxidoreductase (CrtR)
	g602: C4-methyl sterol oxidase
	g904: Delta-sterol C-methyltransferase
	g989: C-22 sterol desaturase (Cyp61)
GO:0055114	Oxidation-reduction process	409	7	2.19	27	3.6E-03	3.6E-03
	g1377: HMGR
	g3385: SE-domain-containing protein (Squalene epoxidase)
	g5794: Fatty acid hydroxylase
	g5928: Cytochrome P450 oxidoreductase (CrtR)
	g602: C4-methyl sterol oxidase
	g943: NAD-P-binding protein
	g989: C-22 sterol desaturase (Cyp61)
GO:0010142	Farnesyl diphosphate biosynthetic process	1	1	0.01	28	5.4E-03	5.4E-03
	g3516: HMGS

The enrichment analysis was performed with the topGO package, using as input the list of Sre1 direct target genes included in Table 4. Table shows GO terms according to the “classic” and “weight01” algorithms and the “fisher” statistic. Significant GO terms were selected and sorted by the P associated with the algorithm “weight01”.