Fig 5. Effects of juvenile treatments on titrations of ZIKV in Ae. aegypti tissues.

(A) Viral body, (B) leg, and (C) saliva titers (plaque forming unit equivalents/ml) of ZIKV-positive female Ae. aegypti. Horizontal lines indicate the mean of viral titers. Whiskers denote the standard error of the means. Each circle represents the titer for an individual female Ae. aegypti. Open circles in (A) represent the titers for females with non-disseminated infection of ZIKV (i.e., viral infection limited to mosquito midgut), whereas filled circles represent the titers for females with disseminated infection of ZIKV (i.e., viral dissemination from mosquito midgut epithelium). For all treatments, viral body, leg, and saliva titers were estimated at 15 days post-ZIKV infection.