REAGENTS or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CR3022	Peter Kwong, VRC/NIH	ter Meulen et al., 2006; Yuan et al., 2020
B38	Peter Kwong, VRC/NIH	Wu et al., 2020
H4	Peter Kwong, VRC/NIH	Wu et al., 2020
P2B-2F6	Peter Kwong, VRC/NIH	(Ju et al., 2020a)
S309	Peter Kwong, VRC/NIH	Pinto et al., 2020
Chemicals and Recombinant Proteins
M1-Pseudouridine 5'-triphosphate	TriLink	N-1081
CleanCap	TriLink	N-7413
Cellulose	Sigma-Aldrich	11363-250G
MEGAscript™ T7 Transcription Kit	ThermoFisher Scientific	AMB13345
QuikChange Lightning Site-Directed Mutagenesis Kit	Agilent	210518
FreeStyle 293 Expression Medium	GIBCO	12338018
Hyclone SFM4HEK293	Cytiva	SH30521.02
Opti-MEM I	GIBCO	31985-070
Turbo293	Speed BioSystems	PXX1002
8% Glutaraldehyde	Electron Microscopy Sciences	16019
300mesh Cu carbon coated	Electron Microscopy Sciences	CF300-Cu
Uranyl formate	Electron Microscopy Sciences	22450 S-888
Experimental Models: Organisms/strains
BALB/c mouse	Charles Rivers Laboratories	N/A
Rhesus macaques	BioQUAL	N/A
Experimental Models: Cell Lines
293T/ACE2 cells	Drs. Mike Farzan and Huihui Mu at Scripps	293T/ACE2 cells
HEK293T/17	ATCC	N/A
Freestyle 293-F cells	GIBCO	R79007
Recombinant DNA
pTEV-di-proline SARS-CoV-2 Spike-A101	Weissman Lab	N/A
pTEV full length S-furin mutant-A101	Weissman Lab	N/A
pTEV-RBD-A101	Weissman Lab	N/A
VRC7480.D614G	VRC and Montefiori lab	N/A
pCMV ΔR8.2	VRC	Naldini et al., 1996
pHR’ CMV Luc	VRC	Naldini et al., 1996
ραH-S-GSAS/PP	Wrapp et al., 2020	N/A
ραH-S-GSAS	Acharya Lab	N/A
ραH-S-GSAS/D614G	Acharya Lab	N/A
Software and Algorithms
Image Lab	Bio-Rad	Version 6.0
Relion	(Scheres, 2012; Scheres, 2016b)	Version 3.1
UCSF Chimera	(Pettersen et al., 2004)	http://www.cgl.ucsf.edu/chimera/
Data and Software Availablity
Additional Supplemental Items	Mendeley Data	https://doi.org/10.17632/cn5b3hc5zd.1