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. 2020 Dec 1;9:e61672. doi: 10.7554/eLife.61672

Figure 2. Evoked and spontaneous glycinergic input to the IO.

(A) Electrically evoked IPSCs in the DAO with average (top, black) and individual trials (gray), before (left) and after washing of Gbz. (right). (B) Average Gbz.-sensitive IPSC (GABA, black) and strychnine-sensitive IPSC (Gly, red) for cell in A. (C) Properties of GABA and glycine components of evoked release in the DAO, showing the rise and decay kinetics (left) and ratios of glycine and GABA evoked amplitude and charge (right). Markers are individual cells. (D) Top: Example of mIPSCs in a PIO neuron recorded in the presence of strychnine (left). Bottom: Same as in top, but for a different PIO neuron recorded in the presence of Gbz. No events were detected over the course of 10 min. (E) Same as in D, but for two cells in the IOB/A subnuclei. F: Average mIPSCs for cells in E. (G) Plot of amplitude vs. decay time for averaged mIPSCs of all cells across the IO. Each marker is a cell. (H) Color-coded map of glycinergic mIPSC frequencies for all recorded cells in the rostral (left) and caudal (right) IO. Markers are individual cells.

Figure 2—source data 1. Evoked and spontaneous glycinergic input to the IO.

Figure 2.

Figure 2—figure supplement 1. GABA and glycine can be co-released in the IO.

Figure 2—figure supplement 1.

(A) Example of mIPSCs in a DAO cell before (gray) and after washing of Gbz. (red). (B) Top: Amplitude of all events in baseline (gray) and in the presence of Gbz. (red) plotted against their decay time for ten minutes of recording in each condition for cell shown in A. Bottom: Histogram of mIPSC decay time for all events in baseline (black) and in the presence of Gbz. (red). (C) Large amplitude mIPSCs with individual events (light) and average event (dark) before (gray, top) and after wash in of Gbz. (bottom, red) for the cell shown in A and B.