Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | HEK293T | ATCC | CRL-3216 | |
| Cell line (Homo-sapiens) | HEK293TΔADRB2 + Landing Pad | This paper | Construction Information found in Endogenous ADRB2 Deletion using CRISPR/Cas9 and Landing Pad Genome Editing Sections | |
| Gene (Homo-sapiens) | ADRB2 | NCBI | Gene ID 154 | |
| Chemical compound, drug | Isoproterenol | Millipore Sigma | I5627 | |
| Chemical compound, drug | Forskolin | Millipore Sigma | F6886 | |
| Commercial assay or kit | Dual Glo Luciferase Assay | Promega | E2920 | |
| Recombinant DNA reagent | TALEN plasmids | Addgene | #51554 #51555 |
|
| Recombinant DNA reagent | SpCas9 plasmid | Addgene | pX339 | |
| Sequence-based reagent | Oligonucleotide Microarray | Agilent | Custom Synthesis | |
| Commercial assay or kit | Nextseq Mid Output 300 cycle | Illumina | 20024905 | |
| Commercial assay or kit | Nextseq High Output 75 cycle | Illumina | 20024906 | |
| Strain, strain background (Escherichia coli) | Dh5 alpha | New England Biolabs | C2989K | |
| Antibody | AlexaFluor 488 Anti-Flag rat monoclonal | Thermo Fisher | MA1-142-A488 | (1:100) |
| Transfected construct (Homo-sapiens) | ADRB2 barcoded variant-reporter library | This paper | Reagent Construction Information found in Variant Library Generation and Cloning Section | |
| Commercial assay or kit | RNEasy Miniprep Kit | Qiagen | 74104 | |
| Commercial assay or kit | Plasmid Plus DNA Maxi Kit | Qiagen | 12963 | |
| Commercial assay or kit | Superscript IV | Thermo Fisher | 18091050 | |
| Commercial assay or kit | Lipofectamine 3000 | Thermo Fisher | L3000001 | |
| Commercial assay or kit | D1000 DNA Screen Tape | Agilent | 5067–5582 | |
| Commercial assay or kit | D1000 Reafents | Agilent | 5067–5583 | |
| Commercial assay or kit | SYBR FAST QPCR Master Mix | Roche | 07959362001 | |
| Commercial assay or kit | Zymo Clean Gel DNA Recovery Kit | Zymo Research | D4007 | |
| Commercial assay or kit | Zymo DNA Clean and Concentrator Kit | Zymo Research | D4013 | |
| Chemical compound, drug | CD293 | Thermo Fisher Scientific | 11913019 | |
| Software, algorithm | BBTools | Brian Bushnell | https://jgi.doe.gov/data-and-tools/bbtools/ | |
| Software, algorithm | Jensen-Shannon Conservation | https://doi.org/10.1093/bioinformatics/btm270 | ||
| Software, algorithm | OMA Orthology Database | https://doi.org/10.1093/nar/gkx1019 | ||
| Software, algorithm | FreeSASA | 10.12688/f1000research.7931.1 | ||
| Software, algorithm | EVmutation | doi:10.1038/nbt.3769 | ||
| Software, algorithm | Parasail | http://dx.doi.org/10.1186/s12859-016-0930-z |