Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Catsper1 | GenBank | Gene ID: 225865 | |
| Gene (Mus musculus) |
Catsper1 | GenBank | Gene ID: 225865 | |
| Strain, strain background (Escherichia coli) | 10-b | New England BioLabs | Cat# C3019H | |
| Strain, strain background Mus musculus |
B6.129S4-Catsper1tm1Clph/J | Laboratory of David E. Clapham (Ren et al., 2001) |
RRID:IMSR_JAX:0
18311 |
|
| Strain, strain background Mus musculus |
B6D2-Tg(CAG/Su9 -DsRed2,Acr3-EGFP)RBGS002Osb/OsbRbrc |
Hasuwa et al., 2010 | RRID:IMSR_RBRC03743 | |
| Strain, strain background Mus musculus |
C57BL/6 | Charles River Laboratories | Crl:029 | |
| Strain, strain background Mus musculus |
B6D2F1 | Charles River Laboratories | Crl:099 | |
| Strain, strain background Mus musculus |
CD1 | Charles River Laboratories | Crl:CD1(ICR) RRID:IMSR_CRL:022 |
|
| Strain, strain background (Escherichia coli) | b-10 | New England BioLabs | CMC0016 | |
| Cell line (Homo sapiens) |
293T | ATCC | Cat# CRL-3216; RRID:CVCL_0063 |
|
| Biological sample (Mus musculus) | Testes | Chung lab, Yale School of Medicine |
N/A | |
| Biological sample (Mus musculus) | Spermatozoa | Chung lab, Yale School of Medicine |
N/A | |
| Antibody | Rabbit polyclonal anti-mCatSper1 | Laboratory of David E. Clapham (Ren et al., 2001) |
RRID:AB_2314097 | Western blot, (2 μg/mL); Immunocytochemistry, (10 μg/mL); In situ imaging, (7 μg/mL) |
| Antibody | Rabbit polyclonal anti-mCatSper3 | Laboratory of David E. Clapham (Qi et al., 2007) |
N/A | (2 μg/mL) |
| Antibody | Rabbit polyclonal anti-mCatSperε | Laboratory of Jean-Ju Chung (Chung et al., 2017) |
N/A | (1.6 μg/mL) |
| Antibody | Rabbit Polyclonal CA-IV antibody | SantaCruz | Cat# sc-25598 | (1:500) |
| Antibody | Mouse monoclonal anti-caveolin1 (clone 2297) | BD Biosciences | Cat# 610406 RRID:AB_397788 |
(1:500) |
| Antibody | Mouse monoclonal anti-phosphotyrosine (clone 4G10) | EMD milipore | Cat# 05–321; RRID:AB_309678 |
Western blot, (1:1,000); Immunocytochemistry, (1:1000) |
| Antibody | Mouse monoclonal anti-acetylated tubulin (clone 6-11b-1) | EMD milipore | Cat# 7451; RRID:AB_609894 |
Western blot, (1:20,000); In situ imaging, (1:100) |
| Antibody | Rabbit monoclonal anti-beta actin (clone 13E5) | Cell signaling technology | Cat# 4970 RRID:AB_2223172 |
(1:100) |
| Antibody | Rabbit monoclonal anti-HA (clone C29F4) | Cell signaling technology | Cat# 3724 RRID:AB_1549585 |
(1:2,000) |
| Antibody | Goat polyclonal anti-mouse IgG-HRP | Jackson ImmunoResearch |
Cat# 115-035-003; RRID:AB_10015289 |
(1:10,000) |
| Antibody | Goat polyclonal anti-rabbit IgG-HRP | Jackson ImmunoResearch |
Cat# 111-035-144; RRID:AB_2307391 |
(1:10,000) |
| Antibody | Goat polyclonal anti-rabbit IgG-Alexa 568 | Invitrogen | Cat# A-11036 RRID:AB_10563566 |
Immunocytochemistry, (1:1000); In situ imaging, (1:500) |
| Antibody | Goat polyclonal anti-rabbit IgG-Alexa 647 | Invitrogen | Cat# A-21245 RRID:AB_2535813 |
Immunocytochemistry, (1:1000); In situ imaging, (1:500) |
| Antibody | Goat polyclonal anti-mouse IgG-Alexa 488 | Invitrogen | Cat# A-11029 RRID:AB_138404 |
Immunocytochemistry, (1:1000); In situ imaging, (1:500) |
| Antibody | Goat polyclonal anti-mouse IgG-Alexa 647 | Invitrogen | Cat# A21236 RRID:AB_2535805 |
Immunocytochemistry, (1:1000); In situ imaging, (1:500) |
| Antibody | Mouse monoclonal anti-HA agarose (clone HA-7) | EMD milipore | Cat# A2095 AB_257974 |
(10 uL) |
| Antibody | Anti-HA magnetic beads (clone 2–2.2.14) | Thermo scietific | Cat# 88836 RRID:AB_2749815 |
(5 uL) |
| Recombinant DNA reagent | pcDNA3-mCatsper1 | Laboratory of David E. Clapham |
N/A | |
| Recombinant DNA reagent | pcDNA3.1(-)-Flag-Catsper1-FL-HA | This paper | N/A | Encoding Mouse CatSper1, 1–686 aa |
| Recombinant DNA reagent | pcDNA3.1(-)-Flag-Catsper1-ND-HA | This paper | N/A | Encoding Mouse CatSper1, 345–686 aa |
| Commercial assay or kit | O-glycosidase | New England BioLabs | Cat# P0733S | (80,000 Unit) |
| Commercial assay or kit | PNGase F | Sigma-Aldrich | Cat# P9120 | (2 nM Unit) |
| Peptide, recombinant protein | Protein phosphatase 1 (PP1) | New England BioLabs | Cat# P0754 | (0.1 Unit) |
| Peptide, recombinant protein | Protein tyrosine phosphatase 1B (PTP1B) | Abcam | Cat# Ab42574 | (5 Unit) |
| Chemical compound, drug | Sodium orthovanadate | New England BioLabs | Cat# P0758S | (1 mM) |
| Chemical compound, drug | H89 | Calbiochem | Cat# 371963 | (50 μM) |
| Chemical compound, drug | ST-Ht31 | Promega | Cat# V8211 | (10 μM) |
| Chemical compound, drug | Calyculin A | Calbiochem | Cat# 208851 | (100 nM) |
| Chemical compound, drug | A23187 | Sigma-Aldrich | Cat# C7522 | (10 μM) |
| Chemical compound, drug | Calpain inhibitor I | Calbiochem | Cat# 208719 | (20 μM) |
| Chemical compound, drug | Calpain inhibitor II | Enzo life science | Cat# BML-PI100 | (20 μM) |
| Chemical compound, drug | Calpain inhibitor III | Enzo life science | Cat# BML-PI130 | (20 μM) |
| Chemical compound, drug | PNA-Alexa 568 | Invitrogen | Cat# L32458 | (1:500) |
| Chemical compound, drug | WGA-Alexa 555 | Invitrogen | Cat# W32464 | (1:500) |
| Chemical compound, drug | WGA-Alexa 647 | Invitrogen | Cat# W32466 | (1:500) |
| Chemical compound, drug | Azo-initiator | Wako | Cat# VA-044 | |
| Chemical compound, drug | 32% Paraformaldehyde | Electron Microscopy Sciences | Cat# 15714 s | |
| Chemical compound, drug | 50% Glutaraldehyde | Electron Microscopy Sciences | Cat# 16310 | |
| Chemical compound, drug | Electrophoretic Tissue Clearing Solution | Logos Biosystems | Cat# C13001 | |
| Chemical compound, drug | N-methyl-d-glucamine | Sigma-Aldrich | Cat# M2004 | |
| Chemical compound, drug | Diatrizoic acid | Sigma-Aldrich | Cat# D9268 | |
| Chemical compound, drug | 60% Iodixanol | Sigma-Aldrich | Cat# D1556 | |
| Chemical compound, drug | DAPI | Thermo scietific | Cat# D1306 | 1:1000 |
| Chemical compound, drug | Hoechst | Thermo scietific | Cat# 62249 | 1 μg/mL |
| Chemical compound, drug | Acrylamid | BioRad | Cat# 1610140 | |
| Software, algorithm | ImageJ | National Institue of Health | Public domain: https://imagej.nih.gov/ij/ | |
| Software, algorithm | Zen software | Carl Zeiss AG | ZEISS ZEN lite www.zeiss.com | |
| Software, algorithm | Imaris | BitPlane; Oxford instruments | Imaris 9.6 | |
| Software, algorithm | MATLAB | MathWorks | MATLAB 9.3 (R2017b) | |
| Software, algorithm | Blender | Blender Foundation, community | Blender 2.79 www.blender.org |
|
| Software, algorithm | GraphPad | GraphPad Software | GraphPad Prism 5 | |
| Software, algorithm | ANNs development source materials | This paper | Source archive attached to Figure 5 | Set of ANN development tools |
| Software, algorithm | CatSper quadrilateral structure 3D in situ analytical tools | This paper | Source archive attached to Figure 6 | Set of 3D CatSper analytical tools |