Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Other | IGHA_MOUSE | UniProtKB RRID:SCR_004426 |
UniProtKB: P01878 | Amino acid sequence of Mus musculus IgA heavy chain constant regions |
| Other | LAC1_MOUSE | UniProtKB RRID:SCR_004426 |
UniProtKB: P01843 | Amino acid sequence of Mus musculus lambda-1 light chain constant region |
| Other | IGJ_MOUSE | UniProtKB RRID:SCR_004426 |
UniProtKB: P01592 | Amino acid sequence of Mus musculus joining chain |
| Other | PIGR_MOUSE | UniProtKB RRID:SCR_004426 |
UniProtKB: O70570 | Amino acid sequence of Mus musculus polymeric Ig Receptor |
| Other | STA121 | Moor et al., 2017 | Variable region of human IgA2 antibody STA121 | |
| Cell line (Homo sapiens) | HEK Expi293F | Thermo Fisher RRID:SCR_008452 |
Cat#: A14635 RRID:CVCL_D615 |
|
| Recombinant DNA reagent | pD2610-v1 | ATUM | Cat#: pD2610-v1-03 | Mammalian expression plasmid |
| Transfected construct (Homo sapiens) | STA121 IgA Heavy chain (HC) in pD2610v1 | Materials and methods of this paper | Construct encoding STA121 heavy chain (HC) variable region fused to Mus musculus IgA HC constant domains | |
| Transfected construct (Homo sapiens) |
STA121 IgA light chain (LC) lambda in pD2610v1 | Materials and methods of this paper | Construct encoding STA121 light chain (LC) variable region fused to Mus musculus lambda LC constant domain | |
| Transfected construct (Homo sapiens) |
Mouse Secretory component (SC) in pD2610v1 | Materials and methods of this paper | Construct encoding residues 1–567 of Mus musculus pIgR (a.k.a. secretory component; SC) | |
| Transfected construct (Homo sapiens) |
Mouse joining chain (JC) in pD2610v1 | Materials and methods of this paper | Construct encoding Mus musculus joining chain (JC) |
|
| Peptide, recombinant protein | STA121 Secretory IgA | Materials and methods of this paper | Protein complex produced from transfected constructs and containing: STA121 HC, STA121 LC, JC, SC | |
| Peptide, recombinant protein | STA121 dimeric IgA | Materials and methods of this paper | Protein complex produced from transfected constructs and containing: STA121 HC, STA121 LC, JC | |
| Other | CaptureSelect LC-lambda (Mouse) Affinity Matrix | Thermo Fisher RRID:SCR_008452 |
Cat#: 194323005 | Affinity matrix for protein purification |
| Other | Superose 6 Increase 10/300 GL | GE Healthcare Life Sciences RRID:SCR_000004 |
Cat#: 29091596 | Size exclusion column for protein purification |
| Software, algorithm | Rosetta CryoEM refinement package | Wang et al., 2016 | RRID:SCR_015701 | |
| Software, algorithm | Phenix | Afonine et al., 2018a; Afonine et al., 2018b | Phenix RRID:SCR_014224
Phenix.refine RRID:SCR_016736 |
|
| Software, algorithm | Pymol Molecular Graphics System | Schrodinger LLC RRID:SCR_014879 |
RRID:SCR_000305 | |
| Software, algorithm | RELION-3 | Scheres, 2012; Zivanov et al., 2018 | RRID:SCR_016274 | |
| Software, algorithm | UCSF Chimera | Pettersen et al., 2004; Yang et al., 2012 | RRID:SCR_004097 | |
| Software, algorithm | UCSF ChimeraX | Goddard et al., 2018 | RRID:SCR_015872 | |
| Software, algorithm | cryoSPARC v.2 | Punjani et al., 2017 | RRID:SCR_016501 | |
| Software, algorithm | VMD | Humphrey et al., 1996 | RRID:SCR_001820 |