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. 2020 Dec 1;9:e60226. doi: 10.7554/eLife.60226

Figure 3. Treatment with BPTES increases incorporation of carbons from glutamine into endogenously synthesized amastigote sterols.

(A) Table of detectable isolated amastigote sterol species from and the percentage of natural sterols (i.e. without detectable 13C). The proportion of species found with the indicated number of incorporated 13C carbons are shown (e.g. 13C1, 13C2). (B) Normalized area (ISTD) of the indicated species without and without BPTES treatment.

Figure 3.

Figure 3—figure supplement 1. BPTES does not inhibit oxygen consumption of isolated amastigotes from glutamine and sensitizes amastigotes to ketoconazole.

Figure 3—figure supplement 1.

(A) Measurement of the oxygen consumption rate (OCR) of isolated amastigotes when glutamine is the sole carbon source. BPTES was injected three times, increasing the BPTES concentration by 5 μM per injection for a final well concentration of 15 µM. (B) Dose response curves of glutamine in the presence of ketoconazole and at various indicated concentrations of BPTES.