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. 2020 Sep 10;10(3):2045894020948470. doi: 10.1177/2045894020948470

Fig. 1.

Fig. 1.

Upregulated protein expression of PDGFRα and PDGFRβ in PASMC from patients with IPAH. (A) Representative Western blot images (a) and summarized data (b, means ± SE) showing the protein expression of PDGFRα, PDGFRβ, pAKT at S473 (pAKT-S473) and at T308 (pAKT-T308), and total AKT in PASMC isolated from normal subjects (blue bars, n = 3 subjects) and IPAH patients (red bars, n = 5 patients). *p < 0.05, ***p < 0.001 versus normal. (B) Representative Western bolt images (a) and summarized data (b, means ± SE) showing the protein expression of PDGFRα and PDGFRβ in IPAH-PASMC transfected with control siRNA (si-Control) and siRNA specifically targeting PDGFRα (si-PDGFRα) and PDGFRβ (si-PDGFRβ). ***p < 0.001 versus si-Control. (C) Representative Western bolt images (a) and summarized data (b, means ± SE) showing the protein expression of PDGFRα, PDGFRβ, pAKT (S473), and total AKT in PASMC and PAEC isolated from normal subjects and IPAH patients. **p < 0.001 versus normal PASMC. (D) Representative images showing immunofluorescence in PASMC (upper panels) and PAEC (lower panels) isolated from normal subjects and IPAH patients stained for PDGFRα (a) and PDGFRβ (b) (green). Nuclei counterstained with DAPI (blue). AKT: protein kinase B; DAPI: 4′,6-diamidino-2-phenylindole; IPAH: idiopathic PAH; PAEC: pulmonary arterial endothelial cell; pAKT: phosphorylated AKT; PASMC: pulmonary arterial smooth muscle cell; PDGFRα: platelet-derived growth factor receptor α; PDGFRβ: platelet-derived growth factor receptor β; siRNA: small interfering RNA.